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Time it takes to run a DNA analysis
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What about the Maury show?
pretty sure they take the DNA sample a ways in advance.
-”Confront the DNA with our database, see if something match”
-”Don’t worry, I’ll make the computer search in our database containing 6 billions humans, we should get the results in.. Oh! Looks like we get it!”
-”Nice, so I can put my sunglass on”
YEAAAAAAAAAAAAAAAAAAAH!!!!!!
First of all, we’re now at seven billion. Keep up. Secondly, CSI is based on a group of American forensics people. Therefore, it only searches America’s DNA databases (and most of those people aren’t in there). Please, keep up.
He was just being funny, why be like that?…
You need ask? It’s because he’s a douche who had his sense of humor removed rectally by a faulty pool filter.
Bad joke > Troll.
No. No, it’s really not.
Actually there have been international killers, especially on CSI Miami, douche
2+ weeks? Yikes, what old tech are you using? Though I guess it depends on what kind of “analysis” you’re running. I run bacterial identification in under 4 hours, including extraction from raw samples.
But I hear ya. Just because I can physically do the test in 4 hours doesn’t mean the client gets their RESULTS REPORT in 4 hours, but they insist on thinking they should, and call me in the lab to ask where their results are. “I mailed them in this morning, how come I don’t have my report yet!??”
4 hours for bacterial identification? I mean PCR takes 3h, and sequencing even more
Do you sequence or do you run an agarose gel? If you’re only amplifying VNTR’s of decent size, say 100 bp or more, you could tell the number of them on an agarose gel.
Sequencing a PCR product – or rather having the sequencing lab churn out one for me rather, usually took about 3 days. But this was 5 years ago, things may have changed since then.
That’s why I said it depends on what kind of analysis you’re doing.
We test samples where we know the bacteria has a high likelihood of being A, B, C, or D, so it’s quicker than trying to test for everything under the sun.
My PCR itself takes 1.5 hours to run, and the rest of the 4 hour window I gave is sample prep and extraction.
After you run the PCR, how do you analyze the product?
After we run RT-PCR, the sample has already been identified based on what channel(s) produced amplification with our known primers, and no further analysis is needed beyond quantification and reporting (unless requested by the client, but they usually don’t want to pay any more than they have to).
Thank you DNA Lab Tech – took the words right out of my mouth. I can tell you if someone has MRSA within four hours, Chlamydia in two!
Gotcha. Didn’t realize you were running RT-PCR.
Yeah, but for bacteria you can use the 454 machine. Takes no time at all.
I agree.
I’ve always wondered why it does supposedly take so long for tests like paternity. I mean don’t you already have the primers worked out as well as the annealing temps? For those tests you’re amplifying the VNTR’s right? At least this is what I did when I was looking for certain markers.
Also I’d think on a buccal swab you don’t even need to extract the DNA. In fact I’ve seen ol’ Greg there just put some EDTA solution (probably) in the microfuge tube along with the swab tip, vortex it, and take a few microliters of that for a template for PCR.
Definitely takes longer than 30 secs, but less than a day I’d think.
…and there you have it folks. The most erudite conversation in graphjam history! I’m actually a statistician, so I personally have nothing of value to add unto this reply stream. I’m just enjoying the ride!
Wow, that’s interesting. I just went and saw the CSI Experience in Vegas last night.
DNA Some times takes Years
Only when the underfunded crime lab gets its budget slashed in half, or your state closes one of their only 3 labs completely. It’s not the test that takes years, it’s the beaurocracy.
The two weeks is mostly shipping the stuff to the lab and back and queueing it up with lots of other tests that need running. The actual analysis time varies according to the technique used, but generally takes at least a couple days as some of the processes require long incubation times.
This is true. PCR/electrophoresis is pretty quick. For sequencing, the running actually only takes a couple of hours – Sanger sequencing is really only a modified PCR/electrophoresis technique after all. But because the sequencing machine runs many samples in parallel, they wait until they have the machine full before running it to save time and consumables. In fact, at one point we had to sequence many thousands of clones – I would fire off about 600 at a time for sequencing, and we got around a 16 hour turnaround because not only did we send enough to fill the machine but also they knew they had an enormously valuable contract they gave us priority 1 time on the machine.
Coincidentally, the whole process of doing such a chore addled my brain enough to go to grad school, of course a decision of debatable wisdom :S
The same is true for any other diagnostic. Mail in a single swab and it’ll get thrown into the process whenever a tech gets around to it, which of course can be weeks. If there’s a reason to do it immediately the turnaround is surprisingly rapid. That simpsons episode where they found the angel isn’t too far off truth. (“Did I say three to five weeks? I meant three to five seconds!”)
I baed the 2+ weeks from a half-remembered comment my sister-in-law (who was doing her Masters in Biochemistry) said when we first started watching CSI, many years ago. *G*
Oh, by the way, commentators, no-one cares if you hate CSI. If you blast it for whatever reason, please, tell me what shows YOU enjoy and I can find just as many arguments for why it sucks (provided I’ve seen it). I am aware that CSI has flaws, but what show/film/book doesn’t? The trick is to look past that.
I enjoy CSI (and CSI: NY). *S*
I like CSI. Never seen the others. XD
Okay, so we’ve determined there’s neither barium nor radium in this sample.
Reference win!
CSI play in an alternate Reality in the future
NCIS ftw., seems more realistic to me
^^^^ pretty much all what I was thinking … even on the crusty old crapnology in my uni lab they were doing classic style (lots of horizontal band) gene testing on an overnight / 24h basis.
What causes the holdup is that you can only do so many of these at once, and sometimes you want to test more than a handful of loci or get a more detailed look, which is when you have to start chopping things up and iterating.
But still… two weeks… that’s a hell of a lot of detail. World’s most potentially-lucrative paternity suit or something?
In a clinical setting I agree, it shouldn’t take that long. However, the organism I research is super GC rich, which means PCR fails A LOT. Apparently in the not too distant past getting PCR to work on this organism was worthy of a doctorate. One time it took me a few months to amplify a gene!
hahaha real time is actually 3-4 months….
You forgot Ace Attorney- 2 secs.